KMS XINJIANG INSTITUTE OF ECOLOGY AND GEOGRAPHY，CAS
| 新疆地区棉铃虫的抗性监测及其交配选择性研究 | |
| 王盼盼 | |
| Subtype | 硕士 |
| Thesis Advisor | 吕昭智 |
| 2017-05-01 | |
| Degree Grantor | 中国科学院大学 |
| Place of Conferral | 新疆乌鲁木齐 |
| Degree Discipline | 工程硕士 |
| Keyword | Bt棉花 棉铃虫 抗性 寄主来源 交配 Bt-cotton Helicoverpa armigera resistance host mating |
| Abstract | Bt-cotton controls Helicoverpa armigera effectively. Single toxin (Cry1Ac) Bt-cottonhas been intensively planted for nearly 15 years in Xinjiang Province, but it is not endorsedby any authority. The status of Cry1Ac susceptibility of H. armigera in this region has notbeen routinely monitored. The same is true for the susceptibility of H. armigera to cottonexpressing Cry2Ab toxin, which has not been released in China. The susceptibility of H.armigera field populations to Cry1Ac and Cry2Ab was assessed in nine populations ofXinjiang in 2014–2015. In order to learn the main host of larva and mating of H. armigera,moths collected from 9 populations were dissected and analyzed by stable carbon isotopein 2014-2015. Moreover, the mating experiment of H.armigera between different resistantlevels and ages was completed in lab from August to December in 2016. The main resultsare as follows:1. The response to Cry1Ac of the nine H. armigera strains ranged from 3.16 to 16.94μg/ml for LC50, 0.013 to 0.741 μg/ml for IC50, and <0.001 to 0.038 μg/ml for EC50 overtwo consecutive years. The susceptibility of H. armigera field populations to Cry1Ac inXinjiang does not appear to have shifted significantly over the past 15 years sinceBt-cotton has been intensive planted. There was no significant difference in susceptibilityto Cry1Ac between strains originating from collections on small-holder versus broad-acrefarms.2. The susceptibility of these strains to Cry2Ab ranged from 3.43 to 19.05 μg/ml for LC50,0.16 to 3.81 μg/ml for IC50, and <0.001 to 0.24 μg/ml for EC50 over two years. There wasno significant difference in susceptibility to Cry2Ab between strains originating fromcollections on small-holder versus broad-acre farms. Pearson correlation analysis was usedto estimate the correlation between Cry1Ac and Cry2Ab responses. Across the nine fieldpopulations, there were no significant correlations between Cry1Ac and Cry2Absusceptibility for any of the three measurements in 2014 or in 2015.3. The host use by H. armigera larvae. The results showed that in different months,female and male larvae had parasitise on different hosts. However, most of them feed on C3 hosts.For Korle, there were 85% female from C3 and 90% male from C3 in 2013, 85%female from C3 and 83.5% male from C3 in 2014, 58% female from C3 and 56% malefrom C3.The mating performance of H.armigerea in Korla. There were always some moths notmating in the field. Mostly, high mating proporations had high mating frenquencies. Basedon stable carbon isotope analysis, in 2013 and 2014, the mating percentage of C3*C4 wereabout 55% and 54% in June, 8% and 9.5% in July, 22% and 28% in August and after,respectively. The mating percentage of C3*C4 was 41% in 2015. The high percentage ofC3*C4 indicated that the H. armigera can mate randomly from different hosts and C4could be a host for H.armigera.4. Mating. The results of multi-choice mating experiment, single-choice matingexperiment and single pairs mating experiment showed there were no significant differenceof the mating ratio, mating frequency and duration time between mating types. Theseindicated that H.armigera between different resistant levels and ages could mate randomly.There were significant difference for the mating between 5-day old male and 1-day oldmale. This indicated that the males’ age could affect their mating choice. |
| Other Abstract | 新疆种植Bt棉（转Cry1Ac毒蛋白）已有15年历史，为了明确棉铃虫长时间在Bt棉的选择压力下抗性水平的变化以及种植模式（兵团和地方）对抗性变化是否有影响，于2014-2015年对新疆不同种植模式下的9个地理种群进行了毒蛋白Cry1Ac和Cry2Ab的敏感性水平测定。为了解田间棉铃虫的主要寄主来源及交配情况，于2014-2015年通过解剖和碳稳定性同位素分析的方法，评估了新疆9个地区棉铃虫的寄主来源，以及库尔勒地区棉铃虫的田间交配情况。并于2016年8-12月完成了实验室条件下不同抗性水平、不同日龄的棉铃虫交配试验，结果表明：1. 新疆地区田间棉铃虫对Cry1Ac 仍处于敏感水平。新疆不同地理种群棉铃虫对Cry1Ac的抗性测定结果分别为3.16~16.94ug/ml（LC50），0.013~0.741ug/ml（IC50），<0.001~0.038 ug/ml（EC50）。新疆地区田间棉铃虫对Cry1Ac的抗性水平呈现上升趋势，但目前仍处于敏感水平，并未产生抗性，且种植模式对棉铃虫的抗性无影响（p>0.05）。2. 新疆不同地理种群棉铃虫对Cry2Ab的敏感基线。新疆不同地理种群棉铃虫对Cry2Ab敏感基线数据分别为3.43~19.05ug/ml（LC50），0.16~3.81ug/ml（IC50），<0.001~0.24ug/ml（EC50）。新疆地区棉铃虫对Cry2Ab毒蛋白仍处于敏感水平。种植模式对棉铃虫的抗性无影响（p>0.05）。毒蛋白Cry1Ac和Cry2Ab之间无相关性。3. 田间棉铃虫寄主来源分析。2014、2015年新疆9个地理种群不同月份、不同性别的棉铃虫寄主来源不同，但大部分棉铃虫来源于C3植物。库尔勒地区棉铃虫雌蛾与雄蛾的寄主来源。2013年和2014年均约有85%的棉铃虫雌蛾来源于C3植物，2015年有58%的雌蛾来源于C3植物，42%的雌蛾来源于C4植物。2013年约有90%的棉铃虫雄蛾来源于C3植物，2014年约有83.5%的棉铃虫雄蛾来源于C3植物；2015年有56%的棉铃虫雄蛾来源于C3植物，44%的棉铃虫雄蛾来源于C4植物。库尔勒地区棉铃虫的交配特征。在棉铃虫的整个发生期，总有一定比例的棉铃虫未交配，不同日期间交配率和交配频率差异较大，但交配率高的日期往往具有较高的交配频率。通过对棉铃虫雌蛾与对应精包的同位素分析，2013年和2014年在6月份C3*C4的交配比例较高，分别为55%、54%，8月份以后C3*C4的交配比例分别为22%、28%。2015年C3*C4的交配比例总和达到了41%，初步证明来自于C3的棉铃虫与来自于C4的棉铃虫能够自由交配。4. 不同抗性水平及日龄的棉铃虫交配。通过多选择交配实验、单选择交配实验和单对单交配实验，各交配类型的交配率、交配次数、交配时间均无显著差异，表明不同抗性水平和日龄的棉铃虫可自由交配。但雄蛾日龄对交配时间（从释放到第一次交配）有显著影响，5-日龄雌蛾*5-日龄雄蛾与 1-日龄雌蛾*5-日龄雄蛾之间交配无差异（p>0.05），但与 5-日龄雌蛾*1-日龄雄蛾之间有差异，表明雄蛾的日龄会影响棉铃虫的交配选择性。 |
| Subject Area | 生物工程 |
| Language | 中文 |
| Document Type | 学位论文 |
| Identifier | http://ir.xjlas.org/handle/365004/14900 |
| Collection | 研究系统_荒漠环境研究室 |
| Affiliation | 中国科学院新疆生态与地理研究所 |
| First Author Affilication | 中国科学院新疆生态与地理研究所 |
| Recommended Citation GB/T 7714 | 王盼盼. 新疆地区棉铃虫的抗性监测及其交配选择性研究[D]. 新疆乌鲁木齐. 中国科学院大学,2017. |
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