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CdTe/CdS/ZnS核壳量子点团聚体的水生生物毒性研究
赵佳玥
学位类型硕士
导师张道勇
2017-05-01
学位授予单位中国科学院大学
学位授予地点新疆乌鲁木齐
学位专业工程硕士
关键词量子点 生物膜 FTIR FRAP quantum dot biofilm
摘要Artificial nanoparticles, includuing quantum dots, have been highlighted in recent years because of their mechanical properties, reactivity, photoelectric properties, and telex conductivity, which are widely used in pharmacy, materials, biotechnology and environmental protection. A large number of nanoparticles will enter into the environment inevitably, such as atmosphere, aquatic and soil through a variety of ways, which would trigger the awareness about environmental safety and health. And the status would change constantly, for example, from dispersion, agglomeration, sedimentation, to suspension. This paper mainly concerned the toxicity of CdTe/CdS/ZnS Core-Shell-Shell quantum dot aggregates to typical organisms in the aquatic environment, such as biofilms, aglae and zebrafishes. It could be separated into three aspects. First, the adsorption and diffusion process of CdTe/CdS/ZnS Core-Shell-Shell quantum dot aggregates in biofilms and its toxicity to biofilms, mainly using FTIR (Total Internal Reflection Fluorescence), FRAP(Fluorescence Recovery After Photobleaching), and 3D reconstruction. Second, the toxicity of CdTe/CdS/ZnS Core-Shell-Shell quantum dot aggregates on Euglena gracilis, including the morphological changes of algae, cholorophyll fluorescence, pigment synthesis, motility and surface properties of algae under the effect of humic acid and CdTe/CdS/ZnS Core- Shell-Shell quantum dot aggregates. Third, the toxicity of CdTe/CdS/ZnS Core-Shell-Shell quantum dot aggregated to zebrafish in the presence of transparent extracellular polymeric particles, CLSM as the main instrument, and several probes are used as well, such as Phen Green SK (diacetate) and CM-H2DCFDA, to detect the accumulation of quantum dots in2+different tissues and organs of zebrafish, DNA damage, the presence of intracellular Cd2+and Zn and ROS as well. The main conclusions are as follows. (1) C. testoteroni could adsorb aggregates of the CdTe/CdS/ZnS quantum dot ( 1 µm), they could further diffuse into deeper layers of the biofilm and penetrate the 45 µm thick biofilm within 25 min. The amount of accumulated quantum dots in microlayers of the biofilm decreased linearly with depth. FRAP results showed that about 30% fluorescence intensity recovered within 5 min, indicating the mobility of quantum dots in the biofilm. The quantum dots dissolved in the biofilm and would release heavy metals and finally exert toxic effects on the biofilms.(2) CdTe/CdS/ZnS Core-Shell-Shell quantum dot could accumulate in algae, the cellswere transformed from strip to sphere. And cells would lose their athletic ability and reunite together further. The photosynthesis Fv/Fm decreased significantly in a short time, indicating quantum dots affected the physiological activity of photosynthesis. The content of chlorophyll a and carotene in the cells treated by quantum dots decreased, while the cynthesis of chlorophyll b stayed the same. The content of polysaccharide and protein decreased after threatened by quantum dots, and the peaks of carboxyl were slightly increased, which could be caused by the combination of carboxyl groups on the surface of quantum dots and the algae. (3) CdTe/CdS/ZnS Core-Shell-Shell quantum dot aggregates could cause DNA damage to gill and intestines cell of zebrafish, and accumulate in some organs and tissues, such as gills, intestines and muscle. When TEP existed, quantum dots accumulate in above-mentioned organs less. The CdTe/CdS/ZnS Core-Shell-Shell quantum dots could release2+ 2+Cd and Zn in gill and liver cells, which could induce the oxidative stress reactions further, while TEP could remit it. But most of the qutumn dots could be excreted out of bodies withtime.
其他摘要本文讨论研究CdTe/ZnS核壳量子点团聚体对水生环境中典型的生物体……
学科领域环境工程
语种中文
文献类型学位论文
条目标识符http://ir.xjlas.org/handle/365004/14914
专题研究系统_荒漠环境研究室
作者单位中国科学院新疆生态与地理研究所
第一作者单位中国科学院新疆生态与地理研究所
推荐引用方式
GB/T 7714
赵佳玥. CdTe/CdS/ZnS核壳量子点团聚体的水生生物毒性研究[D]. 新疆乌鲁木齐. 中国科学院大学,2017.
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