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学科主题: 生物学::生物化学与分子生物学
题名:
Binding of dicamba to soluble and bound extracellular polymeric substances (EPS) from aerobic activated sludge: A fluorescence quenching study
作者: Pan ; Xiangliang ; Liu ; Jing ; Zhang ; Daoyong ; Chen ; Xi ; Song ; Wenjuan ; Wu ; Fengchang
通讯作者: Zhang, DY, Chinese Acad Sci, Inst Geochem, State Key Lab Environm Geochem, Guiyang 550002, Guizhou, Peoples R China
关键词: BOVINE SERUM-ALBUMIN ; DISSOLVED ORGANIC-MATTER ; ANAEROBIC SLUDGE ; HUMIC ACIDS ; SPECTROSCOPY ; ADSORPTION ; EXTRACTION ; EXCITATION ; LIGANDS
刊名: JOURNAL OF COLLOID AND INTERFACE SCIENCE
发表日期: 2010
卷: 345, 期:2, 页:442-447
收录类别: SCI
部门归属: [Liu, Jing; Zhang, Daoyong] Chinese Acad Sci, Inst Geochem, State Key Lab Environm Geochem, Guiyang 550002, Guizhou, Peoples R China; [Pan, Xiangliang; Chen, Xi; Song, Wenjuan] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Xinjiang, Peoples R China; [Wu, Fengchang] Chinese Res Inst Environm Sci, State Environm Protect Key Lab Lake Pollut Contro, Beijing 100012, Peoples R China
摘要: Binding of dicamba to soluble EPS (SEPS) and bound EPS (BEPS) from aerobic activated sludge was investigated using fluorescence spectroscopy. Two protein-like fluorescence peaks (peak A with Ex/Em = 225 nm/342-344 nm and peak B with Ex/Em = 275/340-344 nm) were identified in SEPS and BEPS. Humic-like fluorescence peak C (Ex/Em = 270-275 nm/450-460 nm) was only found in BEPS. Fluorescence of the peaks A and B for SEPS and peak A for BEPS were markedly quenched by dicamba at all temperatures whereas fluorescence of peaks B and C for BEPS was quenched only at 298 K. A dynamic process dominated the fluorescence quenching of peak A of both SEPS and BEPS. Fluorescence quenching of peak B and C was governed a static process. The effective quenching constants (log K-a) were 4.725-5.293 for protein-like fluorophores of SEPS and 4.23-5.190 for protein-like fluorophores of BEPS, respectively. Log K-a for humic-like substances was 3.85. Generally, SEPS had greater binding capacity for dicamba than BEPS, and protein-like substances bound dicamba more strongly than humic-like substances. Binding of dicamba to SEPS and BEPS was spontaneous and exothermic. Electrostatic force and hydrophobic interaction forces play a crucial role in binding of dicamba to EPS. (C) 2010 Elsevier Inc. All rights reserved..
内容类型: 期刊论文
URI标识: http://ir.xjlas.org/handle/365004/9968
Appears in Collections:中国科学院新疆生态与地理研究所(2010年以前数据)_期刊论文

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Pan,Xiangliang,Liu,et al. Binding of dicamba to soluble and bound extracellular polymeric substances (EPS) from aerobic activated sludge: A fluorescence quenching study[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE,2010,345(2):442-447.
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